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recombinant taq dna polymerase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher recombinant taq dna polymerase kit
    Recombinant Taq Dna Polymerase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+dna/Taq+DNA+Polymerase+Kit/10__3390_slash_mps9030089-85-10-15
    Average 99 stars, based on 1 article reviews
    recombinant taq dna polymerase kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Companion cells with high florigen production express other small proteins and reveal a nitrogen-sensitive FT repressor
    Article Snippet: Recombinant DNA reagent , p35S:NIGT1.2, p35S:NIGT1.4 , and p35S:GFP , This study , , See tobacco transient promoter LUC assay in Materials and methods. .. Recombinant DNA reagent , pENTR-/D-TOPO , Invitrogen , Cat# K230020SP , . .. Commercial assay or kit , Dual Luciferase Reporter Assay System , Promega , Cat# E1910 , .

    Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2
    Article Snippet: Antibody , Anti-FLAG M2 Magnetic Beads , Millipore , M8823 RRID: AB_2637089 , for protein purification. .. Recombinant DNA Reagent , pENTR11 , Invitrogen , K253520 , Plasmid (Shuttle vector for Gateway Cloning). .. Recombinant DNA Reagent , pCW57.1 , Addgene , 41393 , Plasmid (Tet. Inducible lentiviral system - gateway cloning), used for generating TERT and TRF2 PTM induced expression system.

    Article Title: Binding proteins to the human thrombin receptor, PAR4
    Article Snippet: Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPM1-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing mammalian cells.

    Article Title: Conduction pathway for potassium through the Escherichia coli pump KdpFABC
    Article Snippet: Strain, strain background ( Escherichia coli ) , BL21 STAR (DE3 ) , Invitrogen, Carlsbad, CA , Protein expression strain , . .. Recombinant DNA reagent , pBAD , Invitrogen, Carlsbad, CA , Empty vector (plasmid) , . .. Recombinant DNA reagent , pET28a-spNW25 , Addgene ID: 173484 , Plasmid for expression of nanodisc scaffolding protein , .

    Article Title: Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity
    Article Snippet: Antibody , Goat-anti rabbit HRP , Thermo Fisher , 31460 , 1:5000 for western. .. Recombinant DNA reagent , pCDNA5/FRT/TO , Thermo Fisher , V652020 , . .. Recombinant DNA reagent , mRFP-mTrbo cloned into pCDNA5/FRT/TO , This paper , Available upon request , Materials and methods.

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. ..

    Article Title: Regulatory nucleic acid molecules for enhancing gene expression in plants
    Article Snippet: .. Sequence analyses of recombinant DNA were performed with a laser fluorescence DNA sequencer (Applied Biosystems, Foster City, CA, USA) using the Sanger technology (Sanger et al., 1977). ..

    Article Title: Ceftriaxone attenuates Poly I:C–induced neuroinflammation in vitro by modulating glutamate transport, synaptic integrity, and immunometabolic reprogramming
    Article Snippet: .. To analyze spine density, primary hippocampal neurons were transfected with recombinant DNA (mApple N1) using Lipofectamine® 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. To visualize spontaneous calcium transients, primary neurons were transfected with the expression plasmid for GCaMP5g (Addgene, Cat# 31788).

    Plasmid Preparation:

    Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2
    Article Snippet: Antibody , Anti-FLAG M2 Magnetic Beads , Millipore , M8823 RRID: AB_2637089 , for protein purification. .. Recombinant DNA Reagent , pENTR11 , Invitrogen , K253520 , Plasmid (Shuttle vector for Gateway Cloning). .. Recombinant DNA Reagent , pCW57.1 , Addgene , 41393 , Plasmid (Tet. Inducible lentiviral system - gateway cloning), used for generating TERT and TRF2 PTM induced expression system.

    Article Title: Conduction pathway for potassium through the Escherichia coli pump KdpFABC
    Article Snippet: Strain, strain background ( Escherichia coli ) , BL21 STAR (DE3 ) , Invitrogen, Carlsbad, CA , Protein expression strain , . .. Recombinant DNA reagent , pBAD , Invitrogen, Carlsbad, CA , Empty vector (plasmid) , . .. Recombinant DNA reagent , pET28a-spNW25 , Addgene ID: 173484 , Plasmid for expression of nanodisc scaffolding protein , .

    Cloning:

    Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2
    Article Snippet: Antibody , Anti-FLAG M2 Magnetic Beads , Millipore , M8823 RRID: AB_2637089 , for protein purification. .. Recombinant DNA Reagent , pENTR11 , Invitrogen , K253520 , Plasmid (Shuttle vector for Gateway Cloning). .. Recombinant DNA Reagent , pCW57.1 , Addgene , 41393 , Plasmid (Tet. Inducible lentiviral system - gateway cloning), used for generating TERT and TRF2 PTM induced expression system.

    Microinjection:

    Article Title: Binding proteins to the human thrombin receptor, PAR4
    Article Snippet: Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPM1-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing mammalian cells.

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. ..

    Transfection:

    Article Title: Binding proteins to the human thrombin receptor, PAR4
    Article Snippet: Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPM1-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing mammalian cells.

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. ..

    Article Title: Ceftriaxone attenuates Poly I:C–induced neuroinflammation in vitro by modulating glutamate transport, synaptic integrity, and immunometabolic reprogramming
    Article Snippet: .. To analyze spine density, primary hippocampal neurons were transfected with recombinant DNA (mApple N1) using Lipofectamine® 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. To visualize spontaneous calcium transients, primary neurons were transfected with the expression plasmid for GCaMP5g (Addgene, Cat# 31788).

    Liposomes:

    Article Title: Binding proteins to the human thrombin receptor, PAR4
    Article Snippet: Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPM1-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing mammalian cells.

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. ..

    Electroporation:

    Article Title: Binding proteins to the human thrombin receptor, PAR4
    Article Snippet: Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPM1-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing mammalian cells.

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. ..

    Transduction:

    Article Title: Binding proteins to the human thrombin receptor, PAR4
    Article Snippet: Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, MD, USA) and/or cellfectin (Gibco, MD, USA), PEG-mediated DNA uptake, electroporation, viral transduction (e.g., using a lentivirus) and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others. .. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), (Sigma), RPM1-1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing mammalian cells.

    Sequencing:

    Article Title: Regulatory nucleic acid molecules for enhancing gene expression in plants
    Article Snippet: .. Sequence analyses of recombinant DNA were performed with a laser fluorescence DNA sequencer (Applied Biosystems, Foster City, CA, USA) using the Sanger technology (Sanger et al., 1977). ..

    Fluorescence:

    Article Title: Regulatory nucleic acid molecules for enhancing gene expression in plants
    Article Snippet: .. Sequence analyses of recombinant DNA were performed with a laser fluorescence DNA sequencer (Applied Biosystems, Foster City, CA, USA) using the Sanger technology (Sanger et al., 1977). ..



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    Image Search Results


    A) Schematic of the UNG locus in the human genome and the regions targeted for disruption with CRISPR/Cas9. B) Validation of UNG knockouts in 293T cells by immunoblotting and in vitro uracil excision assay [S, uracil-containing 43-mer ssDNA substrate; P, cleaved 30-mer ssDNA product; (-) = HED buffer only (negative control); (+) = 5 Units of E. coli uracil-DNA glycosylase (positive control for uracil excision)]. C) Fluorescence microscopy showing that UNG1/2 double-KO is necessary for full inhibition of nuclear UNG activity (scale=200μm; corresponding flow analysis in Supplementary Figure S4C ).

    Journal: bioRxiv

    Article Title: Mitochondrial uracil DNA glycosylase contributes to nuclear base excision repair

    doi: 10.64898/2026.04.30.721890

    Figure Lengend Snippet: A) Schematic of the UNG locus in the human genome and the regions targeted for disruption with CRISPR/Cas9. B) Validation of UNG knockouts in 293T cells by immunoblotting and in vitro uracil excision assay [S, uracil-containing 43-mer ssDNA substrate; P, cleaved 30-mer ssDNA product; (-) = HED buffer only (negative control); (+) = 5 Units of E. coli uracil-DNA glycosylase (positive control for uracil excision)]. C) Fluorescence microscopy showing that UNG1/2 double-KO is necessary for full inhibition of nuclear UNG activity (scale=200μm; corresponding flow analysis in Supplementary Figure S4C ).

    Article Snippet: Negative and positive controls with HED buffer and recombinant E. coli uracil-DNA glycosylase (New England Biolabs M0280S, lot 10272153), respectively, were included.

    Techniques: Disruption, CRISPR, Biomarker Discovery, Western Blot, In Vitro, Excision Assay, Negative Control, Positive Control, Fluorescence, Microscopy, Inhibition, Activity Assay

    Journal: bioRxiv

    Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

    doi: 10.64898/2026.04.22.719657

    Figure Lengend Snippet:

    Article Snippet: Luminex nucleosome panels consisted of the following 601 147 bp DNA-biotinylated nucleosomes (all from EpiCypher): unmodified (rNuc), 16-0006; H4K12 acetylated ([H4K12ac] 2 ), 16-0312; H4 poly-acetylated ([H4K5acK8acK12acK16ac] 2 ), 16-0313; H3 poly-acetylated ([H3K4acK9acK14acK18ac] 2 ), 16-0336; H4 N-terminal tail truncated ([H4ΔN15] 2 ), 16-0018; and trypsin-digested tailless, 16-0027.

    Techniques: Binding Assay, Fluorescence

    The BRD4 tandem bromodomains stably associate with H4 poly-acetylated nucleosomes in biolayer interferometry. ( A-D ) Biolayer interferometry binding assays of ( A ) BD1-2 with H4 poly-acetylated nucleosomes ([H4K5acK8acK12acK16ac] 2 ), ( B ) BD1-2 with unacetylated nucleosomes, ( C ) BD1 alone with H4 poly-acetylated nucleosomes, and ( D ) BD1 alone with unacetylated nucleosomes. Protein concentrations are indicated at the right of each plot. Note the difference in y-axis scale between ( A ) 1 to -0.5 nm and ( B-D ) 0.3 to -0.3 nm. Octet fitting of BD1-2 binding curves to H4 poly-acetylated nucleosomes (12.5, 6.25, and 3.12 μM) using a 2:1 heterogeneous ligand model produced R 2 values >0.98 (Fig. S2). Experiments were performed in triplicate.

    Journal: bioRxiv

    Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

    doi: 10.64898/2026.04.22.719657

    Figure Lengend Snippet: The BRD4 tandem bromodomains stably associate with H4 poly-acetylated nucleosomes in biolayer interferometry. ( A-D ) Biolayer interferometry binding assays of ( A ) BD1-2 with H4 poly-acetylated nucleosomes ([H4K5acK8acK12acK16ac] 2 ), ( B ) BD1-2 with unacetylated nucleosomes, ( C ) BD1 alone with H4 poly-acetylated nucleosomes, and ( D ) BD1 alone with unacetylated nucleosomes. Protein concentrations are indicated at the right of each plot. Note the difference in y-axis scale between ( A ) 1 to -0.5 nm and ( B-D ) 0.3 to -0.3 nm. Octet fitting of BD1-2 binding curves to H4 poly-acetylated nucleosomes (12.5, 6.25, and 3.12 μM) using a 2:1 heterogeneous ligand model produced R 2 values >0.98 (Fig. S2). Experiments were performed in triplicate.

    Article Snippet: Luminex nucleosome panels consisted of the following 601 147 bp DNA-biotinylated nucleosomes (all from EpiCypher): unmodified (rNuc), 16-0006; H4K12 acetylated ([H4K12ac] 2 ), 16-0312; H4 poly-acetylated ([H4K5acK8acK12acK16ac] 2 ), 16-0313; H3 poly-acetylated ([H3K4acK9acK14acK18ac] 2 ), 16-0336; H4 N-terminal tail truncated ([H4ΔN15] 2 ), 16-0018; and trypsin-digested tailless, 16-0027.

    Techniques: Stable Transfection, Binding Assay, Produced

    AlphaFold model suggesting a plausible mode of BRD4-S engagement with an H4 K12 acetylated nucleosome ([H4K12ac] 2 ). ( A ) Top-ranked AlphaFold model prediction of BRD4-S (blue) in complex with an H4K12ac nucleosome (H2A, golden; H2B, tomato; H3, green; H4, violet, Widom 601 DNA, grey). BD1 and BD2 are shaded in darker blue for clarity. ( B ) Rotated (45°) view of the modeled BRD4-S-H4K12ac nucleosome complex. ( C ) Zoom-in of the BD1 acetyl-lysine binding pocket highlighting residues Y97 and N140 positioned near H4K12ac. ( D ) Zoom-in of the BD2 acetyl-lysine binding pocket highlighting residues Y390 and N433 positioned near H4K12ac. This view also shows the relevant placement of the C-terminal BRD4-S region including the BID, ET, and phosphoregulatory sites, in the model.

    Journal: bioRxiv

    Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

    doi: 10.64898/2026.04.22.719657

    Figure Lengend Snippet: AlphaFold model suggesting a plausible mode of BRD4-S engagement with an H4 K12 acetylated nucleosome ([H4K12ac] 2 ). ( A ) Top-ranked AlphaFold model prediction of BRD4-S (blue) in complex with an H4K12ac nucleosome (H2A, golden; H2B, tomato; H3, green; H4, violet, Widom 601 DNA, grey). BD1 and BD2 are shaded in darker blue for clarity. ( B ) Rotated (45°) view of the modeled BRD4-S-H4K12ac nucleosome complex. ( C ) Zoom-in of the BD1 acetyl-lysine binding pocket highlighting residues Y97 and N140 positioned near H4K12ac. ( D ) Zoom-in of the BD2 acetyl-lysine binding pocket highlighting residues Y390 and N433 positioned near H4K12ac. This view also shows the relevant placement of the C-terminal BRD4-S region including the BID, ET, and phosphoregulatory sites, in the model.

    Article Snippet: Luminex nucleosome panels consisted of the following 601 147 bp DNA-biotinylated nucleosomes (all from EpiCypher): unmodified (rNuc), 16-0006; H4K12 acetylated ([H4K12ac] 2 ), 16-0312; H4 poly-acetylated ([H4K5acK8acK12acK16ac] 2 ), 16-0313; H3 poly-acetylated ([H3K4acK9acK14acK18ac] 2 ), 16-0336; H4 N-terminal tail truncated ([H4ΔN15] 2 ), 16-0018; and trypsin-digested tailless, 16-0027.

    Techniques: Binding Assay

    Bromodomain binding pocket mutations in the BRD4-S disrupt breast cancer growth and migration phenotypes. ( A ) Cell growth assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n≥5 independent experiments). Statistical analysis as described in Methods. ( B ) Transwell migration assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n ≥ 3 independent experiments). Statistical significance was calculated by two-tailed t-tests (***p<0.0005, ****p<0.0001). ( C ) Western blots of endogenous BRD4-S depletion and rescue by 3xFLAG-tagged BRD4-S (1-719) constructs in doxycycline-treated MDA-MB-231 cells. Blots were probed with an antibody raised for the N-terminus of BRD4 (BRD4-S; for endogenous ∼100 kDa), M2 FLAG (FLAG), and β-actin (Actin) ( D ) Graphical illustration of putative model for BRD4-S chromatin engagement and regulation of cancer cell growth and migration (BioRender, https://BioRender.com/7dfa8yg ). This model shows multiple possible binding modes of BRD4-S on poly-acetylated nucleosomes that would be difficult to distinguish or determine in biochemical and structural studies, yet could all contribute to recruitment of regulatory complexes and transcriptional control of nearby genes including those for cancer growth and migration.

    Journal: bioRxiv

    Article Title: Tandem bromodomains of BRD4 cooperatively read poly-acetylated nucleosomes to enhance chromatin engagement and regulate breast cancer phenotypes

    doi: 10.64898/2026.04.22.719657

    Figure Lengend Snippet: Bromodomain binding pocket mutations in the BRD4-S disrupt breast cancer growth and migration phenotypes. ( A ) Cell growth assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n≥5 independent experiments). Statistical analysis as described in Methods. ( B ) Transwell migration assay following BRD4-S depletion and complementation with the indicated 3xFLAG-BRD4-S constructs (mean ± SEM; n ≥ 3 independent experiments). Statistical significance was calculated by two-tailed t-tests (***p<0.0005, ****p<0.0001). ( C ) Western blots of endogenous BRD4-S depletion and rescue by 3xFLAG-tagged BRD4-S (1-719) constructs in doxycycline-treated MDA-MB-231 cells. Blots were probed with an antibody raised for the N-terminus of BRD4 (BRD4-S; for endogenous ∼100 kDa), M2 FLAG (FLAG), and β-actin (Actin) ( D ) Graphical illustration of putative model for BRD4-S chromatin engagement and regulation of cancer cell growth and migration (BioRender, https://BioRender.com/7dfa8yg ). This model shows multiple possible binding modes of BRD4-S on poly-acetylated nucleosomes that would be difficult to distinguish or determine in biochemical and structural studies, yet could all contribute to recruitment of regulatory complexes and transcriptional control of nearby genes including those for cancer growth and migration.

    Article Snippet: Luminex nucleosome panels consisted of the following 601 147 bp DNA-biotinylated nucleosomes (all from EpiCypher): unmodified (rNuc), 16-0006; H4K12 acetylated ([H4K12ac] 2 ), 16-0312; H4 poly-acetylated ([H4K5acK8acK12acK16ac] 2 ), 16-0313; H3 poly-acetylated ([H3K4acK9acK14acK18ac] 2 ), 16-0336; H4 N-terminal tail truncated ([H4ΔN15] 2 ), 16-0018; and trypsin-digested tailless, 16-0027.

    Techniques: Binding Assay, Migration, Growth Assay, Construct, Transwell Migration Assay, Two Tailed Test, Western Blot, Control